Scientific program & proceedings

Tuesday, June 5th

Brief introduction – Tom Wiklund (Chairman)

Welcome address – Jorma Mattinen (Rector of Åbo Akademi University)

Presentation of the CRP Theme Programme of the OECD – Rafael Blasco

Opening lecture

Recent developments in the taxonomy of the genus Flavobacterium (Jean-François Bernardet)

Session 1 – Genomics and evolution (Moderator: Eric Duchaud)

Genetic and genomic analysis of Flavobacterium johnsoniae adhesion, motility, and protein secretion (Abhishek Shrivastava, Sampada Kharade, Ryan G. Rhodes, Yongtao Zhu & Mark J. McBride)

Development of genetic manipulation in Flavobacterium columnare (T. Qin, X. L. Zhang, Nan Li, A. H. Li & P. Nie)

Comparative genomics and transcriptomics of Flavobacterium columnare isolates from genomovars I and II (Mark L. Lawrence, Attila Karsi, Hasan C. Tekedar, Michelle Banes & Derek Gibbs)

Modeling evolution of virulence and opportunistic disease dynamics of Flavobacterium columnare (Ilona Merikanto, Veijo Kaitala & Jouni Laakso)

Session 2 – Environmental Flavobacterium species (Moderator: Jean-François Bernardet)

A novel light stimulated Flavobacterium species from an Antarctic saline lake (Feng Shi & John P. Bowman)

Antarctic microbial mats: treasure-troves of new Flavobacterium diversity (Anne Willems, Sophie Verscheure & Karolien Peeters)

Abundance and distribution of genus Flavobacterium in the Baltic Sea; outlook to de novo genomics of brackish-water Flavobacterium (Veljo Kisand, Helen Tammert, Matthias Labrenz & Klaus Jürgens)

Complete genome sequence of Flavobacterium indicum GPSTA100-9T, isolated from warm spring water (Paul Barbier, Jean-François Bernardet & Eric Duchaud)

Potential role of flavobacterial Por secretion system (PorSS) in root colonization and plant defense system stimulation (Max Kolton, Omer Frenkel, Yigal Elad & Eddie Cytryn)

Session 3 – Biofilm and adhesion (Moderator: Mark J. McBride)

Investigation of biofilm control strategies for Flavobacterium johnsoniae-like isolates (Hafizah Y. Chenia)

Correlations of Flavobacterium columnare colony morphotype with predator resistance, growth, biofilm-forming ability and virulence (Ji Zhang, Jouni Laakso, Johanna Mappes & Lotta-Riina Sundberg)

In vitro and in vivo adhesion of Flavobacterium columnare (Cova R. Arias, Wenlong Cai, Stacey LaFrentz & Stephen A. Bullard)

Adhesion and biofilm production of different Flavobacterium psychrophilum colony phenotypes (Jenny Norrgård & Tom Wiklund)

Wednesday, June 6th

Session 4 – Biology and virulence of Flavobacterium (Moderator: Lotta-Riina Sundberg)

The Flavobacterium columnare challenge: host, genomovar and virulence (Craig A. Shoemaker, Benjamin R. LaFrentz & Phillip H. Klesius)

Cost of virulence in fish farm isolates of Flavobacterium columnare in outside host environment (Katja Pulkkinen, Tarmo Ketola, Jouni Laakso & Lotta-Riina Sundberg)

Cytotoxicity of phase varying Flavobacterium psychrophilum cells (Eva Högfors-Rönnholm & Tom Wiklund)

Potential effects of outside host community diversity on population dynamics of Flavobacterium columnare – a modeling study (Jani Anttila, Lasse Ruokolainen & Jouni Laakso)

Different colony morphologies enlighten the way to the tracks of Flavobacterium columnare virulence factors (Reetta K. Penttinen, Jaana K.H. Bamford & Lotta-Riina Sundberg)

Session 5 – Genomic and genetic diversity (Moderator: Mark L. Lawrence)

Genomic diversity of Flavobacterium species (Eric Duchaud, Paul Barbier, Jean-François Bernardet, Christian Michel, Céline Chantry, Christophe Habib, Pierre Nicolas, Valentin Loux, Eduardo Rocha & Marie Touchon)

Nation-wide and local diversity of the fish pathogen Flavobacterium psychrophilum in Japan revealed by MLST analysis (Erina Fujiwara-Nagata, Pierre Nicolas, Céline Chantry-Darmon, Jean-François Bernardet, Mitsuru Eguchi & Eric Duchaud)

Genotyping of Flavobacterium psychrophilum isolates from wild and farmed salmonids in Norway and Chile (Patricia Apablaza, A. Dyrkorn Løland, Ø.J. Brevik, P. Ilardi, J. Battaglia & A. Nylund)

Structure of Flavobacterium psychrophilum populations infecting farmed rainbow trout (Oncorhynchus mykiss) (Krister Sundell, Sirpa Heinikainen, Pierre Nicolas & Tom Wiklund)

Insight into the genetic diversity and population structure of Flavobacterium psychrophilum in France (Charlene Y.J. Siekoula-Nguedia, Guillaume Blanc, Eric Duchaud & Segolene Calvez)

Session 6 – Control strategies and treatment 1 (Moderator: Kim D. Thompson)

Field evaluation of rainbow trout selectively bred for resistance to bacterial cold water disease (Gregory D. Wiens, Timothy D. Leeds, Scott E. LaPatra, Chris Wilson, Wade Cavendar & Caird E. Rexroad III)

Disinfection of unfertilized salmonid eggs: a new method for prevention of vertical transmission of Flavobacterium psychrophilum (Akira Kumagai)

Novel treatment methods of columnaris disease (Lotta-Riina Sundberg, Elina Laanto & Jaana K.H. Bamford)

Selective breeding of food size rainbow trout against Flavobacteriosis (Scott E. LaPatra, Benjamin R. LaFrentz & Richard H. Towner)

In vitro antimicrobial sensitivities of Flavobacterium spp. isolates recovered from UK farmed fish (Ramón Pérez y Pérez de la Ossa, Kim D. Thompson, Alexandra Adams, Roderick Card & David W. Verner-Jeffreys)

Thursday, June 7th

Session 7 – Detection and identification (Moderator: Inger Dalsgaard)

MALDI-TOF MS for the identification and characterization of Flavobacterium isolates from water and environment (N. Streppavara, H. Segner, O. Petrini & Thomas Wahli)

Farmed nelma (Stenodus leucichthys) is susceptible for Flavobacteria (Satu Viljamaa-Dirks & Henry Kuronen)

Flavobacteriosis, a main problem for fish farming in Sweden (Eva Jansson, Anders Alfjorden, Eva Säker, Olga Stephansson, Sofia Lindström & Anna Aspán)

The role of Flavobacterium psychrophilum in Red Mark Syndrome (Matthijs Metselaar, Kim D. Thompson, Richard Paley, Scott E. LaPatra, David Verner-Jeffreys, Sonia J. Lloyd, Douglas R. Call & Alexandra Adams)

Session 8 – Immunization and vaccination (Moderator: Craig A. Shoemaker)

Immunization of rainbow trout against Flavobacteriosis (Scott E. LaPatra)

Development and efficacy of genomovar II vaccines against columnaris disease (Haitham Mohammed, Oscar Olivares-Fuster, Stacey LaFrentz & Cova R. Arias)

Injection vaccination of rainbow trout (Oncorhynchus mykiss) against Flavobacterium psychrophilum (Børge Fredriksen Nilsen, David Gauthier, Hieu Trung Mai, Liv Hungerholdt & Bjørn Brudeseth)

Immersion vaccination of rainbow trout (Oncorhynchus mykiss) against Flavobacterium psychrophilum (Bjørn Brudeseth, David Gauthier, Krister Sundell & Tom Wiklund)

Invasion of green fluorescent protein-tagged Flavobacterium columnare in embryos of zebrafish (Danio rerio) and identification of protective antigens of F. columnare (X. L. Zhang, T. Qin , Z. X. Liu, N. Li & Pin Nie)

Hydrogen peroxide as pre-treatment stressor in experimental immersion challenge of rainbow trout fry with Flavobacterium psychrophilum (Maya M.M. Henriksen, Lone Madsen & Inger Dalsgaard)

Session 9 – Control strategies and treatment 2 (Moderator: Gregory D. Wiens)

Current research on Flavobacterium psychrophilum (Inger Dalsgaard, Maya M.M. Henriksen, Rói H. Christiansen & Lone Madsen)

The use of probiotics to protect fish against rainbow trout fry syndrome (Tiina L. Korkea-aho, Kim D. Thompson, Anna Papadopoulou, Jouni Heikkinen, Atte von Wright, Brian B. Austin & Alexandra Adams)

Pathological, diagnostic, therapeutic and epidemiological aspects of cold water disease in Chile (Marcos Godoy & Ruben Avendaño-Herrera)

Practical measurements to combat Flavobacterium psychrophilum on a rainbow trout farm (Stephan Hofer)

Functional analysis of antibacterial defence mechanisms in homozygous trout lines with different resistance to Flavobacterium psychrophilum infection (Christian Michel, Nicolas Plault, Jean-François Bernardet, Laurent Labbé & Edwige Quillet)

Influence of organic diets and probiotics on an experimental Flavobacterium psychrophilum infection in rainbow trout fry (Lone Madsen, Hans-Christian Ingerslev, Mette Boye & Inger Dalsgaard)

General discussion and closing remarks

Poster presentations

Adherence and biofilm formation of Flavobacterium psychrophilum in the presence of aquarium or lake water (Abel G. Ríos-Castillo, Kim D. Thompson, Alexandra Adams, Mercedes Marín de Mateo & José Juan Rodríguez-Jerez)

Resistance/sensitivity study of Flavobacterium psychrophilum isolates from French fish farming (Ségolène Calvez, Diane-Gaelle Douet, Guillaume Blanc & Patrick Daniel)