InstrumentationThe FluoTime 200 from PicoQuant has the PicoHarp 300E TCSPC module for efficient and fast time-domain life-time measurements. The system is equipped with Glan-Laser polarizers, and has a blue-sensitive fast PMT for efficient detection in the UV range. Our system will have several led or diode lasers for different wavelengths (led 300 nm, led 320 nm, diode 375 nm). The sample compartment has a Peltier temperature control (-50 oC to 100 oC), and data acquisition and analysis is done with the FluoFit Pro software. The system will be optimized for fluorophore life-time and time-resolved anisotropy measurements. The
QuantaMaster™ from Photon Technologies
International is an
easy-to-use, open architecture spectrofluorometer with
high
sensitivity. A continuous high-pressure xenon lamp
provides a
virtual point source broadband excitation illumination.
Excitation and
emission wavelengths are selected by means of
auto-calibrated,
computer-controlled, scanning QuadraScopic™ monochromator
with
continuously variable slits. Temperature control of the
sample is
obtained by a Peltier system (-10 to +90 oC)
and
temperature ramping is possible. Each of our three
instruments has two
separate detection channels, allowing for easy
fluorescence anisotropy
measurements (with Glan-Thompson polarizes in each
excitation and
emission channel). Instrument control and data acquisition
is
completely computer controlled using the Felix32 software.
ISS PC-1 This
spectrofluorometer is modular, compact, and has high
sensitivity. It operates in T-mode and is equipped with
motorized polarizers, and a Peltier Cuvet system allowing
for temperature ramping. Instrument operation and data
analysis is performed via the Vinci software. The LaserStrobe ™ from Photon Technologies International is an L-format cuvette-based lifetime spectrofluorometer capable of making fast and simple fluorescence lifetime measurements from the ultraviolet to the visible, from 100 picoseconds to tens of microseconds, with single or multiple exponential decays. The illumination source is a pulsed nitrogen dye laser. The excitation wavelength selection is by means of various dyes used in the dye laser (in addition to the nitrogen line at 337.1 nm). A frequency doubler allows for excitation wavelengths into the UV region. Emission wavelength selection is by means of a ¼ meter type monochromator. The QuadraCentric™ sample compartment comes standard with a 10 x 10 mm cuvette holder and a Peltier element for temperature control. The light paths are supplied with Glan-Thompson polarizers. The Cary Eclipse is controlled by modular software, which consists of applications tailored to particular tasks. The Cary Eclipse is fitted with manual polarizers (L-format) and a removable plate-reader. The sample compartment has a Peltier temperature controller and stirring. With the Cary Eclipse one can:
The VP-DSC is a sensitive, easy to use differential scanning calorimeter to study samples in solution. Providing fast, accurate transition midpoint (Tm) determination, the VP-DSC is suitable for the study of aqueous lipid and protein dispersions or solutions. In addition, a complete thermodynamic profile can be generated to understand the factors affecting lipid phase behavior, or conformation and stability of proteins. The VP-DSC is controlled by an intelligent user interface (VPViewer™ software) and data analysis is performed with the Origin® data analysis package. The Nano II differential scanning calorimeter is a high-sensitivity instrument from Calorimetry Sciences Corporation (now TA Instruments). The instrument has capillary sample holders for liquid samples. It operates in a fixed-cell power compensation mode, a design giving maximal baseline repeatability. The temperature range is -10oC to 130oC. The Bruker Daltonics HCT-Ultra Ion Trap Mass Spectrometer is equipped with both ESI and Multimode (APCI/ESI) ion sources, and its mass range is m/z 50-6000. Fragmentation (MSn) can be performed automatically up to 5th stage. The sample can be introduced by directly injection (1-2 microL/min) or via the Agilent 1200 Series Rapid Resolution LC System. The responsible person for this instrument is docent Jussi Meriluoto. The Merck/Hitachi LaChrome HPLC system is equipped with a 4-Ch pump (L7100). Sample loading is via an autosampler (L7200), column temperature is controlled with an oven, detection is accomplished with a diode array UV detector (L7485) or with the fluorescence detector (L7455), or both. The instrument is computer controlled with proprietary control and data analysis software. The Malvern Zetasizer Nano S can measure particle sizes in solution (range 0.6 to 6000 nm) using NIBS technology and dynamic light scattering. Molecular weights of macromolecules can be determined in the range 10,000-2x107 Da. The sample holder has effective temperature control and is programmable, which allows for automated size measurements at different temperatures. Automatic
sample
application is a key factor for productivity of the TLC
laboratory. The Camag TLC Autosampler III offers
fully
automatic sample application for qualitative and
quantitative
analyses as well as for preparative separations. It is
suited for
routine use and high sample throughput in mass analysis.
Gradient elution for Thin-Layer Chromatography The AMD-2 can be successfully
employed for reproducible
gradient elution with silica gel as the stationary phase.
The TLC
Scanner
3 is an advanced workstation for densitometric
evaluation of Thin-Layer Chromatograms. It can also be
used for
densitometric measurements of other planar objects, such
as
electrophoresis gels. All functions of the scanner are
controlled by
software. Only the positioning of the object to be
measured is
performed manually.
|
Biochemistry,
Department
of LABORATORY |