Vesa Hytönen

27.5.2005
Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä

The avidin protein family - properties of family members and engineering of novel biotin-binding protein tools

Reviewers:

Professor Pentti Tuohimaa, Department of Medicine, University of Tampere, Tampere

Professor Kari Keinšnen, Department of Biological and Environmental Sciences, University of Helsinki, Helsinki

Opponent:

Professor Hans Söderlund, VTT Biotechnology, VTT, Finland

Custos:

Professor Markku Kulomaa, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä

Publications:

  1. Hytönen VP, Laitinen OH, Airenne TT, Kidron H, Meltola NJ, Porkka E, Hörhä J, Paldanius T, Määttä JA, Nordlund HR, Johnson MS, Salminen TA, Airenne KJ, Ylä-Herttuala S & Kulomaa MS. (2004) Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli. Biochemical Journal 384(Pt 2):385-390.
  2. Hytönen VP, Nyholm TKM, Pentikäinen OT, Vaarno J, Porkka EJ, Nordlund HR, Johnson MS, Slotte JP, Laitinen OH & Kulomaa MS. (2004) Chicken avidin-related protein 4/5 shows superior thermal stability when compared to avidin retaining high affinity to biotin. J Biol Chem 279:9337-9343.
  3. Eisenberg-Domovich Y, Hytonen VP, Wilchek M, Bayer EA, Kulomaa MS & Livnah O.(2005) High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability. Acta Crystallogr. D, 61:528-538.
  4. Hytonen VP, Maatta JA, Nyholm TK, Livnah O, Eisenberg-Domovich Y, Hyre D, Nordlund HR, Horha J, Niskanen EA, Paldanius T, Kulomaa T, Porkka EJ, Stayton PS, Laitinen OH, Kulomaa MS. (2005) Design and construction of highly stable, proteaseresistant chimeric avidins. J. Biol. Chem. 280:10228-10233.