Tuomas Näreoja

08.08.2014
Department of Cell Biology and Anatomy, Faculty of Medicine, University of Turku

Nonspecific binding in sandwich-type immunoassays utilizing nanoparticulate labels

Reviewers:

Docent Kristiina Takkinen, Immunotechnolgy - VTT, Espoo, Finland

Professor Niko Hildebrandt, Nano Bio Photonics, Institute d'Electronique Fondamentale, Université Paris-Sud, Orsay, Paris, France

Opponent:

Professor Hans Tanke, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

Custos:

Professor Pekka Hänninen, Department of Medical of Cell Biology and Anatomy, University of Turku, Turku

Publications:

  1. Näreoja T, Vehniäinen M, Lamminmäki U, Hänninen PE, Härmä H. (2009) Study on nonspecificity of an immuoassay using Eu-doped polystyrene nanoparticle labels. J Immunol Methods 345(1-2):80-9
  2. Näreoja T, Määttänen A, Peltonen J, Hänninen PE, Härmä H. (2009) Impact of surface defects and denaturation of capture surface proteins on nonspecific binding in immunoassays using antibody-coated polystyrene nanoparticle labels. J Immunol Methods 347(1-2):24-30
  3. Näreoja T, Ebner A, Gruber HJ, Taskinen B, Kienberger F, Hänninen PE, Hytönen VP, Hinterdorfer P, Härmä H. (2014) Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay. Anal Bioanal Chem 406(2):493-503
  4. Näreoja T, Lamminmäki H, Härmä H, Hänninen P. (2014) Super-sensitive time-resolved fluoroimmunoassay for TSH utilizing europium (III) nanoparticle labels with reduced nonspecific binding (Submitted)