Merja Niemi

Department of Chemistry, University of Eastern Finland

A Molecular Basis for Antibody Specificity - Crystal Structures of IgE-Allergen and IgG-Hapten Complexes


Professor Markku Kulomaa, IMT, University of Tampere, Tampere, Finland

Docent Anastassios Papgeorgiou, Centre of Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland


Professor Jari Ylänne, Department of Biological and Environmental Science, Unversity of Jyväskylä, Jyväskylä, Finland


Professor Juha Rouvinen, Department of Chemistry, University of Eastern Finland


  1. Niemi M, Jänis J, Jylhä S, Kallio JM, Hakulinen N, Laukkanen ML, Takkinen K & Rouvinen J. (2008) Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine beta-lactoglobulin allergen. Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1;64(Pt 1):25-28.
  2. Niemi M, Jylhä S, Laukkanen ML, Söderlund H, Mäkinen-Kiljunen S, Kallio JM, Hakulinen N, Haahtela T, Takkinen K & Rouvinen J. (2008) Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen. Structure. Nov;15(11):1413-1421.
  3. Kallio JM, Hakulinen N, Kallio JP, Niemi MH, Kärkkäinen S & Rouvinen J. (2009) The contribution of polystyrene nanospheres towards the crystallization of proteins. PLoS ONE 2009;4(1):e4198.
  4. Niemi MH, Takkinen K, Amundsen LK, Söderlund H, Rouvinen J & Höyhtyä M (in press). The Testosterone Binding Mechanism of an Antibody Derived from a Naïve Human scFv Library. J Mol. Recognit.
  5. Niemi MH, Turunen L, Pulli T, Nevanen TK, Höyhtyä M, Söderlund H, Rouvinen J & Takkinen K (submitted) A Structural Insight into the Molecular Recognition of a (-)-?9-Tetrahydrocannabinol and the Development of a Sensitive One-Step, Homogeneous Immunocomplex Based Assay for its Detection.