Barbara Taskinen

14.02.2014
Institute of Biomedical Technology and Biotechnolgy (BioMedTech), University of Tampere

Protein Engineering of Avidin by Rational Design and DNA Family Shuffling

Reviewers:

Professor Kari Airenne, A.I. Virtanen Institute for Molecular Sciences Department of Biotechnology and Molecular Medicine, University of Eastern Finland, Kuopio, Finland

Professor Kalervo Hiltunen, Department of Biochemistry, University of Oulu Finland

Opponent:

Professor Arne Skerra, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Munich, Germany

Custos:

Docent Vesa Hytönen, Institute of Biomedical Technology and Biotechnolgy (BioMedTech), University of Tampere, Finland

Publications:

  1. Niederhauser, B, Siivonen, J, Määttä, JA, Jänis, J, Kulomaa, MS & Hytönen, VP (2012) "DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools", Journal of Biotechnol, vol. 157:1, 38-49.
  2. Taskinen, B, Airenne, TT, Jänis, J, Rahikainen, R, Johnson, MS, Kulomaa, MS & Hytönen, VP, "A novel chimeric avidin with increased thermal stability using DNA shuffling" (Submitted)
  3. Pollheimer, P, Taskinen, B, Scherfler, A, Gusenkov, S, Creus, M, Wiesauer, P, Zauner, D, Schöfberger, W, Schwarzinger, C, Ebner, A, Tampé, R, Stutz, E, Hytönen, VP & Gruber, H (2013) "Reversible biofunctionalization of surfaces with a switchable mutant of avidin", Bioconjugate Chem., vol. 24:10, 1656-1668.
  4. Taskinen, B, Zmurko, J, Ojanen, M, Kukkurainen, S, Parthiban, M, Määttä, JAE, Leppiniemi, J, Jänis, J, Parikka, M, Turpeinen, H, Rämet, M, Pesu, M, Johnson, MS, Kulomaa, MS, Airenne TT & Hytönen, VP (2013) "Zebavidin - An avidin-like protein from zebrafish", PLOS ONE, vol. 8(10) e77207