National Doctoral Programme in
Informational and Structural Biology

The focal point of our research is a multisubunit RNA polymerase (RNAP), the enzyme that carries out the first step in gene expression, synthesis of RNA. All cellular RNAPs are multisubunit enzymes sharing homologous catalytic cores totaling ~2,500 amino acids. By importance, ancienty and conservation among the life kingdoms RNAP rivals the ribosome, yet is considerably less complex and is amenable to kinetic and mechanistic studies in well-defined in vitro systems. We perform wide-scale studies of RNAP catalytic mechanism with emphasis on translocation, a step specific to processive polymerases and motor enzymes. Another related line of our research deals with transcription elongation factors. Understanding the means by which transcription factors modulate RNAP catalytic efficiency and translocation kinetics helps us to unravel the basic mechanism of this complex molecular machine. Finally, RNAP is both an excellent phylogenetic marker for inferring species phylogenies and an attractive paradigm for studying the protein evolution. We employ molecular phylogenetic analyses to study the evolution of protein landscape in catalytically important areas of RNAP, as well as the evolution of other transcription-related proteins and the whole organisms.