Merja Niemi

7.5.2010
Department of Chemistry, University of Eastern Finland

A Molecular Basis for Antibody Specificity - Crystal Structures of IgE-Allergen and IgG-Hapten Complexes

Reviewers:

Professor Markku Kulomaa, IMT, University of Tampere, Tampere, Finland

Docent Anastassios Papgeorgiou, Centre of Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland

Opponent:

Professor Jari Ylänne, Department of Biological and Environmental Science, Unversity of Jyväskylä, Jyväskylä, Finland

Custos:

Professor Juha Rouvinen, Department of Chemistry, University of Eastern Finland

Publications:

  1. Niemi M, Jänis J, Jylhä S, Kallio JM, Hakulinen N, Laukkanen ML, Takkinen K & Rouvinen J. (2008) Characterization and crystallization of a recombinant IgE Fab fragment in complex with the bovine beta-lactoglobulin allergen. Acta Crystallogr Sect F Struct Biol Cryst Commun. Jan 1;64(Pt 1):25-28.
  2. Niemi M, Jylhä S, Laukkanen ML, Söderlund H, Mäkinen-Kiljunen S, Kallio JM, Hakulinen N, Haahtela T, Takkinen K & Rouvinen J. (2008) Molecular interactions between a recombinant IgE antibody and the beta-lactoglobulin allergen. Structure. Nov;15(11):1413-1421.
  3. Kallio JM, Hakulinen N, Kallio JP, Niemi MH, Kärkkäinen S & Rouvinen J. (2009) The contribution of polystyrene nanospheres towards the crystallization of proteins. PLoS ONE 2009;4(1):e4198.
  4. Niemi MH, Takkinen K, Amundsen LK, Söderlund H, Rouvinen J & Höyhtyä M (in press). The Testosterone Binding Mechanism of an Antibody Derived from a Naïve Human scFv Library. J Mol. Recognit.
  5. Niemi MH, Turunen L, Pulli T, Nevanen TK, Höyhtyä M, Söderlund H, Rouvinen J & Takkinen K (submitted) A Structural Insight into the Molecular Recognition of a (-)-?9-Tetrahydrocannabinol and the Development of a Sensitive One-Step, Homogeneous Immunocomplex Based Assay for its Detection.