Juha Määttä

11.6.2010
Institute of Medical Technology (IMT), University of Tampere

Structural and Functional Characterization of Engineered Avidin Proteins

Reviewers:

Professor Reijo Lahti, Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland

Professor Antti Poso, Department of Pharmacy, University of Eastern Finland, Kuopio, Finland

Opponent:

Professor Pirkko Heikinheimo, Institute of Biotechnology University of Helsinki, Helsinki, Finland

Custos:

Professor Markku Kulomaa, IMT, University of Tampere, Tampere, Finland

Publications:

  1. Hytönen VP, Määttä JA, Nyholm TK, Livnah O, Eisenberg-Domovich Y, Hyre D, Nordlund HR, Hörhä J, Niskanen EA, Paldanius T, Kulomaa T, Porkka EJ, Stayton PS, Laitinen OH & Kulomaa MS. (2005) Design and construction of highly stable, protease-resistant chimeric avidins. J Biol Chem. Mar 18;280(11):10228-33.
  2. Hytönen VP, Määttä JA, Niskanen EA, Huuskonen J, Helttunen KJ, Halling KK, Nordlund HR, Rissanen K, Johnson MS, Salminen TA, Kulomaa MS, Laitinen OH & Airenne TT. (2007) Structure and characterization of a novel chicken biotin-binding protein A (BBP-A). BMC Struct Biol. 2007 Mar 7;7:8.
  3. Määttä JA, Airenne TT, Nordlund HR, Jänis J, Paldanius TA, Vainiotalo P, Johnson MS, Kulomaa MS, Hytönen VP. (2008) Rational modification of ligand-binding preference of avidin by circular permutation and mutagenesis. Chembiochem. 2008 May 5;9(7):1124-35.
  4. Määttä JA, Eisenberg-Domovich Y, Nordlund HR, Hayouka R, Kulomaa MS, Livnah O, Hytönen VP, (2010) Chimeric vidin shows good stability in hars chemical conditions - Biochemical analysis and 3D structure. (submitted)