Georgiy Belogurov

19.11.2004
Department of Biochemistry and Food Chemistry, University of Turku, Turku

Pyrophosphate-energized proton pumps: identification of the residues determining K+ requirements and discovery of a Na+-dependent enzyme

Reviewers:

Professor Mark S Johnson, Department of Biochemistry and Pharmacy, Åbo Akademi University, Turku, Finland

Professor Simo Laakso, Department of Chemical Technology, Helsinki University of Technology, Helsinki, Finland

Opponent:

Professor J. Baz Jackson, School of Biosciences, University of Birmingham, Birmingham, U.K.

Custos:

Professor Reijo Lahti, Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland

Publications:

  1. Belogurov GA, Turkina MV, Penttinen A, Vanhatalo S, Baykov AA & Lahti R. (2002) H+-pyrophosphatase of Rhodospirillum rubrum: High-yield expression in Escherichia coli and identification of the cys residues responsible for inactivation by mersalyl. J Biol Chem. 277: 22209-22214.
  2. Belogurov GA & Lahti R. (2002) A Lysine Substitute for K+. A460K mutation elilminates K+ dependence in H+ -pyrophosphatase of Carboxydothermus hydrogenoformans. J Biol Chem 277:49651-49654.
  3. Malinen AM, Belogurov GA, Salminen M, Baykov AA. & Lahti R. (2004) Elucidating the role of conserved glutamates in H+-pyrophosphatase of Rhodospirillum rubrum. J. Biol. Chem. 279: 26811-26816.
  4. Belogurov GA, Malinen AM, Turkina MV, Jalonen U, Rytkönen K, Baykov AA & Lahti R. Membrane-bound pyrophosphatase of Thermotoga maritima requires sodium for activity. Biochemistry (under revision).